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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Maize with modified growth characteristics
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B104(EF1α::AN3)
No
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- Organization: VIB - Flanders Interuniversity Institute for Biotechnology () | BCH-CON-SCBD-42293-1Organization:VIB - Flanders Interuniversity Institute for Biotechnology ()Rijvisschestraat 120 9052 Zwijnaarde, Belgium Tel.: +09 244 66 11,
, BelgiumPhone:Fax: +09 244 66 10,Email: vib@vib.be,Website: www.vib.be,
The genetically modified maize (Zea mays) has modified growth characteristics resulting from the additional expression (overexpression) of GRF-interacting factor 1 (also known as ANGUSTIFOLIA 3). This gene is a transcriptional co-activator that is involved in the regulation of cell proliferation. As a result of the modification, the duration of growth is elongated, which results in the formation of larger plant organs (e.g., larger leaves) and increased biomass. The modified maize also contains a Streptomyces hygroscopicus phosphinothricin N-acetyltransferase gene cassette, which was used as a glufosinate selectable marker during transformation of the parental line. Additionally, the modified plants contain an Escherichia coli 3"(9)-O-aminoglycoside adenyltransferase gene, under the control of a bacterial promotor, which provides resistance to the antibiotics streptomycin and spectinomycin when present in the correct bacterial genetic background. This gene is not expected to be active in this modified maize.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Maize line B104
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pBbm42GW7
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- Agrobacterium-mediated DNA transfer
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0.000 kb
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0.848 kb
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0.864 kb
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1.997 kb
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0.021 kb
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0.684 kb
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0.021 kb
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0.218 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-277106-1 Elongation factor 1 alpha promoter | Brachypodium distachyon (Purple false brome, Stiff brome, Annual false brome)Promoter
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BCH-GENE-SCBD-277136-1 GRF-interacting factor 1 | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Changes in physiology and/or production (Growth rate, Yield)
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-103358-4 AttB λ attachment site | Escherichia coli (ECOLX)Excision-integration site
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
The modified maize contains two active gene cassettes: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) and Zea mays GRF-interacting factor 1 (gif1).
The bar coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator. Due to the nature of the promoter, high levels of constitutive transcription are expected to occur in all plant tissues.
The gif1 coding sequence is under control of Brachypodium distachyon elongation factor 1 alpha promoter and Cauliflower mosaic virus 35S terminator. Due to the nature of the promoter, high levels of constitutive transcription are expected to occur , particularly for leaf, root and spike tissues.
In the experimental line of this maize, vector backbone sequences, including an Escherichia coli 3"(9)-O-aminoglycoside adenyltransferase coding sequence, were inserted into the maize genome. Since the gene has a bacterial promoter, the gene is only expected to be active in bacterial backgrounds (not wihtin this maize).
Note:
The bar coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator. Due to the nature of the promoter, high levels of constitutive transcription are expected to occur in all plant tissues.
The gif1 coding sequence is under control of Brachypodium distachyon elongation factor 1 alpha promoter and Cauliflower mosaic virus 35S terminator. Due to the nature of the promoter, high levels of constitutive transcription are expected to occur , particularly for leaf, root and spike tissues.
In the experimental line of this maize, vector backbone sequences, including an Escherichia coli 3"(9)-O-aminoglycoside adenyltransferase coding sequence, were inserted into the maize genome. Since the gene has a bacterial promoter, the gene is only expected to be active in bacterial backgrounds (not wihtin this maize).
Note:
- The size of the bar-nopaline synthase terminator was indicated as 848 base pairs. However, information was not available regarding the size of each individual genetic element. Thus, the size of both elements was indicated on the protein coding sequence.
- The size of the 3"(9)-O-aminoglycoside adenyltransferase gene indicated on this record is the size associated with the transformation vector.
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- Research
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